MOSH/MOAH in cocoa powder and cocoa mass (LC-GC-FID and GCxGC-FID)

Method ring test P2705-MRT is designed for the quantification of MOSH and MOAH by means of LC-GC-FID or similar methods. The quantification of total MOAH by GCxGC-FID is offered as an additional module.

LOQs of 2 mg/kg (cocoa mass) resp. 1 mg/kg (cocoa powder) related to total MOSH resp. total MOAH are required for this method ring test in accordance with the expected maximum levels for MOAH.

Two test materials are offered:

a spiked sample of cocoa mass (and the corresponding unspiked material), and
a spiked sample of cooca powder (and the corresponding unspiked material).

Spiked levels are within the typical ranges of contaminations of the respective matrix. The results related to the spiked materials are evaluated with respect to the comparability criterion (z-score) as well as with respect to the trueness criterion (70 to 120 % of the spiked level, as far as scientifically reasonable).

P2705-MRT is offered as a method ring test, which means,

the evaluation is not limited to the pure statistical evaluation.
the participants are asked to provide the chromatograms and plots related to the test materials.
the participants are asked to answer a questionnaire related to relevant aspects of the sample preparation and analysis.

The chromatograms as well as the answers related to the questionnaire are summarised in separate parts of the final report. For reasons of fairness, only those participants, which provide details and chromatograms will receive these parts of the report. The data will help to identify shortcomings, built the basis for further discussion and thus help to enhance the quality of the applied method.

Parameters

Test material - cocoa mass

MOSH ≥ C10 to ≤ C16, MOSH > C16 to ≤ C20, MOSH > C20 to ≤ C25, MOSH > C25 to ≤ C35, MOSH > C35 to ≤ C40, MOSH > C40 to ≤ C50, Total MOSH (≥ C10 to ≤ C50), MOAH ≥ C10 to ≤ C16, MOAH > C16 to ≤ C25, MOAH > C25 to ≤ C35, MOAH > C35 to ≤ C50, Total MOAH (≥ C10 to ≤ C50)

Blank material - cocoa mass

Test material - cocoa powder

Blank material - cocoa powder

Additional module (GCxGC-FID)

Cocoa mass TM - Total MOAH (≥ C10 to ≤ C50), Cocoa mass BM - Total MOAH (≥ C10 to ≤ C50), Cocoa powder TM - Total MOAH (≥ C10 to ≤ C50), Cocoa powder BM - Total MOAH (≥ C10 to ≤ C50)

Expected time schedule

Please register before:
01 April 2027

Dispatch of samples:
07 June 2027

Deadline for reporting results:
29 June 2027

Costs of P2705-MRT

Ring test and material	Amount	Price per unit
MOSH/MOAH in cocoa powder and cocoa mass (LC-GC-FID and GCxGC-FID)	–	990 €
Test material - cocoa mass Cocoa mass, spiked with MOSH and MOAH.	50 g	included
Blank material - cocoa mass Cocoa mass, unspiked.	50 g	included
Test material - cocoa powder Cocoa powder, spiked with MOSH and MOAH.	50 g	included
Blank material - cocoa powder Cocoa powder, unspiked.	50 g	included
Additional module (GCxGC-FID) Quantification by GCxGC-FID (total MOAH).		350 €

Note: PROOF-ACS reserves the right to cancel tests in case of less than ten participants.