The test material contains spiked and incurred levels of pyrrolizidine alkaloids.
The scope of the test is defined by
the so-called BfR28 pyrrolizidine alkaloids
- Echimidine (Em) and echimidine-N-oxide (EmN)
- Erucifoline (Er) and erucifoline-N-oxide (ErN)
- Europine (Eu) and europine-N-oxide (EuN)
- Heliotrine (Hn) and heliotrine-N-oxide (HnN)
- Intermedine (Im) and intermedine-N-oxide (ImN)
- Jacobine (Jb) and jacobine-N-oxide (JbN)
- Lasiocarpine (Lc) and lasiocarpine-N-oxide (LcN)
- Monocrotaline (Mc) and monocrotaline-N-oxide (McN)
- Retrorsine (Re) and retrorsine-N-oxide (ReN)
- Senecionine (Sc) and senecionine-N-oxide (ScN)
- Seneciphylline (Sp) and seneciphylline-N-oxide (SpN)
- Senecivernine (Sv) and senecivernine-N-oxide (SvN)
- Senekirkine (Sk)
- Trichodesmine (Td)
as well as by the co-eluting pyrrolizidine alkaloids defined by EFSA (Document D067816/03 ANNEX)
- Indicine and indicine-N-oxide
- Echinatine and echinatine-N-oxide
- Rinderine and rinderine-N-oxide
- Integerrimine and integerrimine-N-oxide
- Heliosupine and heliosupine-N-oxide
- Spartioidine and spartioidine-N-oxide
- Usaramine and usaramine-N-oxide
The aim of the test is
- the identification of the spiked and incurred pyrrolizidine alkaloids.
- the correct quantification of the spiked alkaloids with respect to the trueness criterion (70-120 % recovery of the spiked level).
- the correct quantification of the spiked and incurred alkaloids with respect to the comparability criterion (z-score ≤ |2|).
echimidine (Em), echimidine-N-oxide (EmN), erucifoline (Er), erucifoline-N-oxide (ErN), europine (Eu), europine-N-oxide (EuN), heliotrine (Hn), heliotrine-N-oxide (HnN), intermedine (Im), intermedine-N-oxide (ImN), jacobine (Jb), jacobine-N-oxide (JbN), lasiocarpine (Lc), lasiocarpine-N-oxide (LcN), lycopsamine (La), lycopsamine-N-oxide (LaN), monocrotaline (Mc), monocrotaline-N-oxide (McN), retrorsine (Re), retrorsine-N-oxide (ReN), senecionine (Sc), senecionine-N-oxide (ScN), seneciphylline (Sp), seneciphylline-N-oxide (SpN), senecivernine (Sv), senecivernine-N-oxide (SvN), senkirkine (Sk), trichodesmine (Td), echinatine, echinatine-N-oxide, heliosupine, heliosupine-N-oxide, indicine, indicine-N-oxide, integerrimine, integerrimine-N-oxide, rinderine, rinderine-N-oxide, spartioidine, spartioidine-N-oxide, usaramine, usaramine-N-oxide
Expected time schedule
Please register before:
01 July 2021
Dispatch of samples:
20 September 2021
Deadline for reporting results:
14 October 2021
Costs of P2116-RT
|Ring test and material
||Price per unit
Pyrrolizidine alkaloids in rooibos tea
Test material rooibos tea
Rooibos tea, milled, with spiked and incurred residues of PAs.
Blank material rooibos tea
Rooibos tea, milled, unspiked.
Note: PROOF-ACS reserves the right to cancel tests in case of less than ten