Pyrrolizidine alkaloids in rooibos tea

The test material contains spiked and incurred levels of pyrrolizidine alkaloids.

The scope of the test is defined by

the so-called BfR28 pyrrolizidine alkaloids

  • Echimidine (Em) and echimidine-N-oxide (EmN)
  • Erucifoline (Er) and erucifoline-N-oxide (ErN)
  • Europine (Eu) and europine-N-oxide (EuN)
  • Heliotrine (Hn) and heliotrine-N-oxide (HnN)
  • Intermedine (Im) and intermedine-N-oxide (ImN)
  • Jacobine (Jb) and jacobine-N-oxide (JbN)
  • Lasiocarpine (Lc) and lasiocarpine-N-oxide (LcN)
  • Monocrotaline (Mc) and monocrotaline-N-oxide (McN)
  • Retrorsine (Re) and retrorsine-N-oxide (ReN)
  • Senecionine (Sc) and senecionine-N-oxide (ScN)
  • Seneciphylline (Sp) and seneciphylline-N-oxide (SpN)
  • Senecivernine (Sv) and senecivernine-N-oxide (SvN)
  • Senekirkine (Sk)
  • Trichodesmine (Td)

as well as by the co-eluting pyrrolizidine alkaloids defined by EFSA (Document D067816/03 ANNEX)

  • Indicine and indicine-N-oxide
  • Echinatine and echinatine-N-oxide
  • Rinderine and rinderine-N-oxide
  • Integerrimine and integerrimine-N-oxide
  • Heliosupine and heliosupine-N-oxide
  • Spartioidine and spartioidine-N-oxide
  • Usaramine and usaramine-N-oxide

The aim of the test is

  • the identification of the spiked and incurred pyrrolizidine alkaloids.
  • the correct quantification of the spiked alkaloids with respect to the trueness criterion (70-120 % recovery of the spiked level).
  • the correct quantification of the spiked and incurred alkaloids with respect to the comparability criterion (z-score ≤ |2|).


echimidine (Em), echimidine-N-oxide (EmN), erucifoline (Er), erucifoline-N-oxide (ErN), europine (Eu), europine-N-oxide (EuN), heliotrine (Hn), heliotrine-N-oxide (HnN), intermedine (Im), intermedine-N-oxide (ImN), jacobine (Jb), jacobine-N-oxide (JbN), lasiocarpine (Lc), lasiocarpine-N-oxide (LcN), lycopsamine (La), lycopsamine-N-oxide (LaN), monocrotaline (Mc), monocrotaline-N-oxide (McN), retrorsine (Re), retrorsine-N-oxide (ReN), senecionine (Sc), senecionine-N-oxide (ScN), seneciphylline (Sp), seneciphylline-N-oxide (SpN), senecivernine (Sv), senecivernine-N-oxide (SvN), senkirkine (Sk), trichodesmine (Td), echinatine, echinatine-N-oxide, heliosupine, heliosupine-N-oxide, indicine, indicine-N-oxide, integerrimine, integerrimine-N-oxide, rinderine, rinderine-N-oxide, spartioidine, spartioidine-N-oxide, usaramine, usaramine-N-oxide

Expected time schedule

Please register before:
01 July 2021
Dispatch of samples:
20 September 2021
Deadline for reporting results:
14 October 2021

Costs of P2116-RT

Ring test and material Amount Price per unit
Pyrrolizidine alkaloids in rooibos tea 790 €
Test material rooibos tea
Rooibos tea, milled, with spiked and incurred residues of PAs.
50 g included
Blank material rooibos tea
Rooibos tea, milled, unspiked.
50 g included

Note: PROOF-ACS reserves the right to cancel tests in case of less than ten participants.